Associate Scientist/Research Associate

Summary

Analytical Associate Scientist with three years of industry experience in cystic fibrosis research and epithelial electrophysiology.  Extensive experience with industry-standard and novel cell-based assays for the study of epithelial function.  Designed and executed mission-critical experiments, especially equivalent current assays on primary airway cells using the TECC apparatus, including data analysis.  Presented experimental data at lab-wide meetings; produced detailed documentation for all protocols and experiments.  Responsible for primary airway cell culture, lab equipment maintenance, procurement and general lab operations.  Highly motivated self-starter and excellent communicator, seeking a position to pursue my interest in genetic disease and further my career goals in the biotechnology industry.    

Education

Rochester Institute of Technology, Rochester, NY

Bachelor of Science in Biotechnology (May 2010)

  • Graduated with highest honors; GPA 3.9; Minor in Music
  • Honors: Golden Key National Honors Society, Dean’s List

Experience

Pfizer, Cambridge, MA

Associate Scientist, Cystic Fibrosis                                                                                         2013 to Present

Research Highlights:

  • Performed cell-based assays to study the effects of small molecules on CFTR trafficking and function, including the TECC Equivalent Current assay.
  • Developed new protocols to improve quality of data and increase productivity of Equivalent Current assay.
  • Extensively analyzed data from multiple assay formats of Equivalent Current.
  • Protein quantification using BCA and Western Blot analysis.
  • Cultured primary human bronchial epithelial cells, NIH/3T3 cells and Fischer rat thyroid cells.
  • Presented scientific research and results to senior staff and at company-wide scientific meetings.

 

Flatley Discovery Lab, Charlestown, MA  

Research Associate II, Electrophysiology                                                                                   2010 to 2013

Research Highlights:

  • Performed cell-based assays to study the effects of small molecules on CFTR trafficking and function, including the TECC Equivalent Current assay and the Ussing Chamber short circuit current assay.
  • Lead the implementation, installation, and validation of the company’s Equivalent Current assay.
  • Developed new protocols and new assays to improve quality of data and increase productivity.
  • Ussing Chamber knowledge includes growing, washing, and treating cells, experimental compound additions, cleaning, breakdown, and storage, as well as data analysis and presentation.
  • Extensively analyzed data from multiple assays including Ussing Chamber and Equivalent Current.
  • Cultured primary human bronchial epithelial cells, NIH/3T3 cells and Fischer rat thyroid cells.
  • Presented scientific research and results to senior staff and at company-wide scientific meetings.
  • Responsible for ordering, receiving, and maintaining lab supplies, compounds and stocks.

Publications

Liang, F., Kwok, I., Layer, E., Harrington, J., Valdez, R., Bhatt, P., Amarnani, D., Sui, J., Cole, B., Bihler, H., Mense, M., Fitzpatrick, R. (2011). Lead Identification of CFTR-F508del Correctors Through a Combination of Functional and Mechanistic Assays. Poster presentation delivered at North American Cystic Fibrosis Conference, Anaheim, CA, October, 2011.

Liamg, F., Kwok, I., Bhatt, P., Kaczmarek, S., Layer, E., Valdez, R., Fitzpatrick, R., Kolodziej, A. (2012). RDR-1 Corrects DF508-CFTR in Synergy with VX-809, but Independent of an Interaction with DF508-NBD1. Poster Presentation will be delivered at North American Cystic Fibrosis Conference, Orlando, FL, October, 2012.

Shang, H., Li, T., Kanawade, A., Layer, E., Valdez, R., Garg, B., Green, O., Kaczmarek, S., Kolodziej, A., Fitzpatrick, R., Sui, J. (2012). From Multiple Cell Line High Throughput Screening to the Rescue of ΔF508-CFTR in Primary hBE Cells. Poster Presentation delivered at North American Cystic Fibrosis Conference, Orlando, FL, October, 2012.

  • ID#: 73550
  • Location: Bellevue, WA , 98004

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